The iodoform test is used to detect the presence of a methyl ketone group (CH3-CO-) or a group that can be oxidised to it, such as CH3-CH(OH)-. When such a compound is warmed with iodine and sodium hydroxide (or sodium hypoiodite), it gives a yellow precipitate of iodoform (CHI3) with a characteristic smell. Compounds like acetaldehyde, acetone, ethanol and isopropanol give a positive iodoform test.

Amines are derivatives of ammonia in which one, two or three hydrogen atoms are replaced by alkyl or aryl groups. They are classified as: primary (1 degree) amine, in which one hydrogen of ammonia is replaced (e.g. CH3NH2, methylamine); secondary (2 degree) amine, in which two hydrogens are replaced (e.g. (CH3)2NH, dimethylamine); and tertiary (3 degree) amine, in which all three hydrogens are replaced (e.g. (CH3)3N, trimethylamine).

The Hinsberg test uses Hinsberg's reagent (benzenesulphonyl chloride, C6H5SO2Cl). A primary amine reacts to form a sulphonamide that is soluble in alkali (because the N-H hydrogen is acidic). A secondary amine forms a sulphonamide that is insoluble in alkali (it has no N-H hydrogen left to ionise). A tertiary amine does not react at all with the reagent (it has no replaceable hydrogen on nitrogen). Thus the three classes of amine are distinguished by their behaviour with Hinsberg's reagent.

The carbylamine reaction (isocyanide test) is a reaction in which a primary amine (aliphatic or aromatic) is heated with chloroform and alcoholic potassium hydroxide to form an isocyanide (carbylamine), which has an extremely unpleasant (foul) smell: R-NH2 + CHCl3 + 3KOH -> R-NC + 3KCl + 3H2O. Secondary and tertiary amines do not give this reaction. Because only primary amines respond, the carbylamine reaction is used as a test to detect primary amines.

R-NH2 + CHCl3 + 3KOH -> R-NC + 3KCl + 3H2O

Aniline is less basic than ethylamine (and than ammonia) because in aniline the lone pair of electrons on the nitrogen atom is delocalised (drawn) into the benzene ring by resonance. As a result the lone pair is less available for donation to a proton, so aniline accepts a proton less readily and is a weaker base. In ethylamine, the alkyl group has an electron-releasing (+I) effect that increases the electron density on nitrogen and makes the lone pair more available, so it is more basic.

The coupling reaction is a reaction in which a diazonium salt reacts with an electron-rich aromatic compound such as phenol or an aromatic amine to form a brightly coloured azo compound (containing the -N=N- linkage). For example, benzenediazonium chloride reacts with phenol in a mildly alkaline medium to give p-hydroxyazobenzene (an orange dye). These coupling reactions are used to prepare a large number of azo dyes.

Based on their behaviour on hydrolysis, carbohydrates are classified as: (1) monosaccharides - simple sugars that cannot be hydrolysed further into smaller units (e.g. glucose, fructose); (2) oligosaccharides - which give 2 to 10 monosaccharide units on hydrolysis, the most common being disaccharides (e.g. sucrose, which gives glucose and fructose, and maltose); and (3) polysaccharides - which give a large number of monosaccharide units on hydrolysis (e.g. starch, cellulose and glycogen).

A reducing sugar is a carbohydrate that has a free aldehyde or ketone group (a free hemiacetal), so it can reduce Tollens' reagent and Fehling's solution; all monosaccharides (glucose, fructose) and many disaccharides (maltose, lactose) are reducing sugars. A non-reducing sugar has no free aldehyde or ketone group (the reducing groups of both units are involved in the glycosidic bond), so it does not reduce Tollens' or Fehling's reagents; sucrose is the common example of a non-reducing sugar.

Evidence for an aldehyde (-CHO) group in glucose: (1) it reduces Tollens' reagent to give a silver mirror and Fehling's solution to give a red precipitate; (2) it reacts with hydroxylamine to form an oxime and adds one molecule of HCN to form a cyanohydrin, confirming a carbonyl group. Evidence for alcohol (-OH) groups: glucose reacts with acetic anhydride to form a penta-acetate, showing the presence of five -OH groups. Thus glucose is a polyhydroxy aldehyde.

(1) Starch is a polymer of alpha-glucose units and consists of two components, amylose (a linear chain) and amylopectin (a branched chain), joined by alpha-glycosidic linkages; cellulose is a straight-chain polymer of beta-glucose units joined by beta-glycosidic linkages. (2) Starch is the main storage carbohydrate (food reserve) in plants and can be digested by humans, whereas cellulose is a structural material (the main component of plant cell walls) and cannot be digested by humans (we lack the enzyme cellulase).

(1) Primary structure - the specific sequence in which the amino acids are linked in the polypeptide chain. (2) Secondary structure - the local folding of the chain into shapes such as the alpha-helix or beta-pleated sheet, held together by hydrogen bonds. (3) Tertiary structure - the overall three-dimensional folding of the whole polypeptide chain, giving a globular or fibrous shape. (4) Quaternary structure - the arrangement and association of two or more polypeptide chains (subunits), as in haemoglobin.

Enzymes are biological catalysts that speed up the biochemical reactions occurring in living organisms; chemically almost all enzymes are proteins (globular proteins). Characteristics: (1) they are highly specific, each enzyme usually catalysing only one particular reaction or type of reaction (lock-and-key specificity); and (2) they are highly efficient and work best under mild conditions of an optimum temperature (around body temperature) and an optimum pH; they are denatured and lose activity outside these conditions.

Vitamins are organic compounds required in small amounts in the diet for normal growth and health. They are classified into two groups: (1) fat-soluble vitamins (A, D, E and K), which are soluble in fats and oils and are stored in the liver and fatty tissues; and (2) water-soluble vitamins (the B group and C), which are soluble in water and must be supplied regularly in the diet as they are not stored. Deficiency of vitamin C causes scurvy, and deficiency of vitamin D causes rickets in children.

(1) DNA (deoxyribonucleic acid) contains the sugar deoxyribose, whereas RNA (ribonucleic acid) contains the sugar ribose. (2) DNA contains the nitrogenous bases adenine, guanine, cytosine and thymine, whereas RNA contains adenine, guanine, cytosine and uracil (uracil replaces thymine). (3) DNA is usually a double-stranded helix and stores the genetic (hereditary) information, whereas RNA is usually single-stranded and mainly takes part in protein synthesis.