Why do scientists prefer restriction enzymes that leave 'sticky ends' when making recombinant DNA?
Many restriction enzymes cut the two DNA strands in a staggered way, leaving short single-stranded overhangs called sticky ends.
Many restriction enzymes cut the two DNA strands in a staggered way, leaving short single-stranded overhangs called sticky ends. If the vector and the foreign DNA are cut with the same enzyme, their sticky ends have complementary sequences, so they base-pair and hold together. DNA ligase then seals them into one recombinant molecule. Blunt ends join far less efficiently, so sticky ends make recombining DNA much easier.
Key point
Matching sticky ends from the same enzyme base-pair complementarily, letting vector and insert join before ligation.
Common mistake
thinking any two cut DNA pieces will join regardless of the enzyme used.
Memory tip
cut the vector and insert with the SAME enzyme -> matching sticky ends -> easy joining.
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