NEET Biology
NEETBiologyBiotechnology - Principles and ProcessesClass 12

I keep getting “Restriction enzymes” questions wrong in Biotechnology - Principles and Processes. What's the trap?

thinking they cut DNA at random positions.

The usual trap: thinking they cut DNA at random positions. How to avoid it: a restriction enzyme cuts at its own specific palindrome -> matching sticky ends. Go back to the definition — molecular scissors that cut DNA at a specific palindromic sequence, often leaving single-stranded sticky ends.

Key point

Restriction enzymes

Common mistake

thinking they cut DNA at random positions.

Memory tip

a restriction enzyme cuts at its own specific palindrome -> matching sticky ends.

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