NEET · Biology · Biotechnology - Principles and Processes doubts
10 doubts found
Why do scientists prefer restriction enzymes that leave 'sticky ends' when making recombinant DNA?
Many restriction enzymes cut the two DNA strands in a staggered way, leaving short single-stranded overhangs called sticky ends.
How does Bt cotton kill bollworms without being poisonous to humans?
Bt cotton carries a cry gene from Bacillus thuringiensis that makes a protoxin -- an inactive crystal protein.
What does “Restriction enzymes” actually mean in Biotechnology - Principles and Processes, and why does it matter for NEET?
molecular scissors that cut DNA at a specific palindromic sequence, often leaving single-stranded sticky ends.
I keep getting “Restriction enzymes” questions wrong in Biotechnology - Principles and Processes. What's the trap?
thinking they cut DNA at random positions.
What does “Cloning vector” actually mean in Biotechnology - Principles and Processes, and why does it matter for NEET?
a carrier, usually a bacterial plasmid, that ferries recombinant DNA into a host cell.
I keep getting “Cloning vector” questions wrong in Biotechnology - Principles and Processes. What's the trap?
confusing the vector with the host cell.
What does “PCR” actually mean in Biotechnology - Principles and Processes, and why does it matter for NEET?
amplifies DNA in a tube using primers, dNTPs and heat-stable Taq polymerase across denaturation-annealing-extension cycles.
I keep getting “PCR” questions wrong in Biotechnology - Principles and Processes. What's the trap?
thinking PCR needs living cells to copy DNA.
What is the difference between cloning vector and host cell?
The vector (a plasmid or virus) carries the foreign gene; the host cell (like E. coli) takes up the vector and multiplies, producing many copies of the gene.
How is Biotechnology - Principles and Processes tested in NEET, and what should I focus on?
Prioritise the core ideas: Restriction enzymes, Cloning vector, PCR.